Comparison of the Effectiveness of Two Histochemical Staining Techniques for Steatosis Detection in Liver Tissue and Application in Forensic Autopsy: A Case Study

Oil Red O (ORO) stain is lipophilic for fat and lipid components, it one of the chemical reagents which have potential to be utilized forensic medicine identify asphyxia deaths from pulmonary emboli, that are frequently occurred following an accident or sudden death. Consequently, can increase pathologists' confidence lower cost delivering ORO stains other units. Therefore, authors were interested in comparing effectiveness commercial ORO-C homemade ORO-HM steatosis samples. Tissue samples collected divided into two groups namely with fixative without any preservative.Assessment persistence efficiency at different storage periods was performed by four experts a blind testing method. The results compared assessing histochemical using SPSS software. showed both fresh formalin-fixed tissues represented decent fatty changed detection line ORO-C. However, data formalin clearer than tissue (p < 0.05). This study presents method expresses promising results, useful diagnosis as same ORC-C.Furthermore, should slides no longer 72 h time because dye would deteriorate significantly. In conclusion, this provided compatible PRC-C could